A section should first be cut as thin as possible
by a fine saw. It should be attached by the flattest side to a piece of
glass, and then ground down by a grindstone or by very fine emery, on a
perfectly flat piece of lead. When sufficiently thin and transparent,
mount in rectified spirits or Dammar. Sections of the tongue may be made
by embedding in paraffin, and mounted in Farrant's solution or glycerine.
Sections of the stomach may also be made by embedding in paraffin, but
better ones can be made by freezing. Farrant's solution makes a good
mounting.
The intestines also give a better section from freezing than by
embedding, as the paraffin injures the villi; mount in the same medium as
the stomach.
The liver may be embedded in paraffin, and the section mounted in
Farrant's solution or glycerine. The kidney may be treated in the same
way. The cornea of the eye can be readily cut by embedding in paraffin,
and the section may be mounted in Farrant's solution. The crystalline
lens and retina may be treated similarly.
The brain and spinal cord should be embedded in paraffin or a carrot, and
the section mounted in Dammar. Sections of the uterus and ovaries are
best mounted in glycerine or Dammar.
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